iBiology Techniques
iBiology Techniques
  • Видео 445
  • Просмотров 6 533 356

Видео

Next Generation Sequencing 2: Illumina NGS Sample Preparation - Eric Chow (UCSF)Next Generation Sequencing 2: Illumina NGS Sample Preparation - Eric Chow (UCSF)
Next Generation Sequencing 2: Illumina NGS Sample Preparation - Eric Chow (UCSF)
Просмотров 154 тыс.3 года назад
www.ibiology.org/techniques/next-generation-sequencing Next generation sequencing allows DNA samples to be sequenced quickly and affordably. Learn how next gen sequencing works and get tips on preparing and running your samples. In the past decade there has been an amazing change in the efficiency of DNA sequencing. Using traditional Sanger sequencing, the human genome project took 20 years and...
Single Cell Sequencing - Eric Chow (UCSF)Single Cell Sequencing - Eric Chow (UCSF)
Single Cell Sequencing - Eric Chow (UCSF)
Просмотров 235 тыс.3 года назад
www.ibiology.org/techniques/single-cell-sequencing Dr. Eric Chow gives an overview of single cell sequencing, explains why this approach is useful, and talks through the leading methods. Single cell sequencing, as the name implies, allows researchers to examine the genomic information for individual cells. This provides an opportunity to examine cell-to-cell differences and identify cell subtyp...
Bioimage Analysis - Christian Tischer (EMBL)Bioimage Analysis - Christian Tischer (EMBL)
Bioimage Analysis - Christian Tischer (EMBL)
Просмотров 12 тыс.4 года назад
www.ibiology.org/techniques/introduction-to-bioimage-analysis Dr. Christian Tischer walks us through the main concepts of a typical bioimage analysis workflow and explains how to quantitatively interpret the content of microscopy images. Microscopy is a key technology driving biological discovery. Nowadays, microscopy based scientific findings must be substantiated by quantitative image analysi...
Bioimage Analysis 1: The Basics: Getting Started (Anne Carpenter and Kevin Eliceiri)Bioimage Analysis 1: The Basics: Getting Started (Anne Carpenter and Kevin Eliceiri)
Bioimage Analysis 1: The Basics: Getting Started (Anne Carpenter and Kevin Eliceiri)
Просмотров 9 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Image Acquisition for Quantitative Analysis - Nico Stuurman (UCSF)Image Acquisition for Quantitative Analysis - Nico Stuurman (UCSF)
Image Acquisition for Quantitative Analysis - Nico Stuurman (UCSF)
Просмотров 10 тыс.4 года назад
www.ibiology.org/techniques/image-acquisition Nico Stuurman provides an overview of the different tools, equipment, and software available to acquire an image of a biological sample, and the considerations one needs to take when using these tools. How do we visualize biological samples? In this talk, Dr. Nico Stuurman provides an overview of the different tools, equipment, and software availabl...
Bioimage Analysis 2: Pre-Processing (Kevin Eliceiri)Bioimage Analysis 2: Pre-Processing (Kevin Eliceiri)
Bioimage Analysis 2: Pre-Processing (Kevin Eliceiri)
Просмотров 10 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Bioimage Analysis 5: Measurement and Phenotype Classification (Anne Carpenter)Bioimage Analysis 5: Measurement and Phenotype Classification (Anne Carpenter)
Bioimage Analysis 5: Measurement and Phenotype Classification (Anne Carpenter)
Просмотров 7 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Bioimage Analysis 4: Tracking (Kevin Eliceiri)Bioimage Analysis 4: Tracking (Kevin Eliceiri)
Bioimage Analysis 4: Tracking (Kevin Eliceiri)
Просмотров 3,5 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Bioimage Analysis 3: Segmentation (Anne Carpenter)Bioimage Analysis 3: Segmentation (Anne Carpenter)
Bioimage Analysis 3: Segmentation (Anne Carpenter)
Просмотров 34 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Bioimage Analysis 6: Tips and Best Practices (Anne Carpenter and Kevin Eliceiri)Bioimage Analysis 6: Tips and Best Practices (Anne Carpenter and Kevin Eliceiri)
Bioimage Analysis 6: Tips and Best Practices (Anne Carpenter and Kevin Eliceiri)
Просмотров 2,8 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Multi-Dimensional Microscopy Datasets - Loic Royer (CZ Biohub)Multi-Dimensional Microscopy Datasets - Loic Royer (CZ Biohub)
Multi-Dimensional Microscopy Datasets - Loic Royer (CZ Biohub)
Просмотров 4,2 тыс.4 года назад
www.ibiology.org/techniques/multi-dimensional-microscopy-datasets Modern microscopy produces large multi-dimensional datasets, which creates new challenges for data storage, processing and visualization. In this talk, Dr. Loic Royer uses a developing drosophila melanogaster embryo to explain how to solve some of the challenges produced by multi-dimensional microscopy datasets. Speaker Biography...
Metadata in BioImaging - Jason Swedlow (U. Dundee)Metadata in BioImaging - Jason Swedlow (U. Dundee)
Metadata in BioImaging - Jason Swedlow (U. Dundee)
Просмотров 3,6 тыс.4 года назад
www.ibiology.org/techniques/metadata Jason Swedlow explains what constitutes image metadata, and provides examples on how to catalog, organize, analyze, and share the metadata of biological images. In order to understand an image of a biological sample and what it represents, one needs to understand its metadata. Metadata is the information behind the image that shows the experimental procedure...
iBiology Bioimage Analysis Course - Life cycle of an image data setiBiology Bioimage Analysis Course - Life cycle of an image data set
iBiology Bioimage Analysis Course - Life cycle of an image data set
Просмотров 4,5 тыс.4 года назад
www.ibiology.org/online-biology-courses/bioimage-analysis-course This course is designed as a graduate student level introduction to bioimage analysis and will provide an overview of the practice and principles of microscopy digital image handling. This series follows the life cycle of an image data set, from acquisition to analysis. It teaches important concepts and best practices, and provide...
The Scientific Community Image Forum - Anne Carpenter and Kevin EliceiriThe Scientific Community Image Forum - Anne Carpenter and Kevin Eliceiri
The Scientific Community Image Forum - Anne Carpenter and Kevin Eliceiri
Просмотров 5 тыс.4 года назад
www.ibiology.org/techniques/scientific-community-image-forum The Scientific Community Image Forum is an online resource that helps scientists answer their bioimage analysis questions. In this talk, Dr. Anne Carpenter and Dr. Kevin Eliceiri encourage scientists to use the Scientific Community Image Forum when they have image analysis difficulties, and to familiarize themselves with the different...
ImageJ - Kevin Eliceiri (U. Wisconsin)ImageJ - Kevin Eliceiri (U. Wisconsin)
ImageJ - Kevin Eliceiri (U. Wisconsin)
Просмотров 14 тыс.4 года назад
www.ibiology.org/techniques/image-j In this talk, Dr. Kevin Eliceiri provides an overview of ImageJ, explains how ImageJ has evolved through time, and demonstrates major functionalities of this open-source software. Since 1987, different versions of ImageJ have been used by scientists to analyze biological images. Speaker Biography: Dr. Kevin Eliceiri is an Associate Professor of Medical Physic...

Комментарии

  • @higgsbosonberg4316
    @higgsbosonberg4316 6 дней назад

    Video cuts off right when it was getting good

  • @randnorberg4518
    @randnorberg4518 10 дней назад

    RIP roger. May your legacy continue to fluoresce.

  • @SwanOnChips
    @SwanOnChips 11 дней назад

    Both the removal of scaffolding and the protein insertion machines as well as all the other unnamed irreducible complexity and interdependencies scream out Wonderfully Designed. Yet without any scientific evidence or explanation attributes their existence to the god of the evolutionary gaps, 'it evolved'. Has anyone ever explained a naturalistic process that could actually engineer this - or do we really have to rely on that oft repeated story?

  • @GiangTran-u6s
    @GiangTran-u6s 12 дней назад

    Thank you very much for the tutorial! Can it be used for detecting cells in 3D images?

  • @ArtAutumn
    @ArtAutumn 14 дней назад

    Isn't there a mistake on 9:27? I think the Fourier image should be rotated on 90 degrees, coz in the direction of x there are many freaquences due to sharp steps.

  • @Hiblackman
    @Hiblackman 15 дней назад

    Very interesting about nothing.

  • @alankhalil1646
    @alankhalil1646 22 дня назад

    great video. I needed this!

  • @chemistmashwani483
    @chemistmashwani483 23 дня назад

    sir i need a little help in selecting probe for my research can u please help me by ur kind suggestion

  • @zerdofish9989
    @zerdofish9989 25 дней назад

    FACS sorting hehe

  • @harishv7245
    @harishv7245 26 дней назад

    Great explanation professor

  • @gamertcell8425
    @gamertcell8425 27 дней назад

    The very first virus

  • @terpichore7667
    @terpichore7667 Месяц назад

    This was crummy. There are many logical gaps in the presentation. E.g., it is not clear how clusters have the same sequence. There are other RUclips videos on NGS that are clearer.

  • @durgaprasadhembram9662
    @durgaprasadhembram9662 Месяц назад

    Great explanation 👌

  • @CalvinGhasemi
    @CalvinGhasemi Месяц назад

    I found this video very interesting. I have experience with mass spectrometer (LCMS) but this was interesting as it uses a similar principle.

  • @laxmidevi-rf9qj
    @laxmidevi-rf9qj Месяц назад

    kon kon class 8 me h or kis kis ko ye video goggle se mila h like karo

  • @kenschwarz8057
    @kenschwarz8057 Месяц назад

    What a revelation! That Huygens figured out the fundamentals in 1690 is also mind-blowing. Thank you for this fascinating presentation. It utterly changed the way I think about light and optics.

  • @makemyburdenlight
    @makemyburdenlight Месяц назад

    Can someone help me? I read a study that said they "looked at the various forms of the gene coding for a vasopressin receptor" among various people. Does that mean they only looked at active genes, or inactive ones as well?

  • @JamesVestal-dz5qm
    @JamesVestal-dz5qm Месяц назад

    How can you quantitate your images is vitally important because cellular processes are governed by the laws of physics. To help people with biology we need a physically accurate picture of the cell. Fluorescence excitation causes changes in the eigenstate of the electronic energy levels of the excited molecules, which can result in radiation that changes the state of neighboring molecules. However, fluorescence excitation still remains a powerful tool for measuring the rates of cellular processes. Dr. Dray always concludes her videos with sunscreen and subscribe because all humans are in a race against time to slow down the dynamics of radiation causing protein and DNA mutation in their cells.

  • @cary3345
    @cary3345 Месяц назад

    Skip to 14:00 if you just care about microscope

  • @jeannelorraineamponin6822
    @jeannelorraineamponin6822 Месяц назад

    I just want to say that you're a great teacher! Passing on not only knowledge but also the passion!

  • @BruinChang
    @BruinChang Месяц назад

    Thanks a lot to give this presentation!

  • @victoronuche2405
    @victoronuche2405 Месяц назад

    How do i import images on cellprofiler and how can i get a software that works on windows 7

  • @Andrea-sh9sn
    @Andrea-sh9sn Месяц назад

    Thanks a lot. What would happen if instead of putting 5% PhiX in the cartridge, I mistakenly put more amount (60%). And I put the correct amount from the library?

  • @user-ec6mo4sj4z
    @user-ec6mo4sj4z Месяц назад

    amazing lifesaver

  • @esan120au
    @esan120au 2 месяца назад

    Thanks so much! Even the illumina webinar was not as clear as your presentation! 👏👏👏👏

  • @Me-ld8bt
    @Me-ld8bt 2 месяца назад

    Thank you for the great explaination!

  • @nia2121
    @nia2121 2 месяца назад

    this is great!

  • @ggk9828
    @ggk9828 2 месяца назад

    Is it safe then to assume that molecules that divide would be the point at which inorganic becomes organic and chemistry becomes biology?

  • @esan120au
    @esan120au 2 месяца назад

    Thanks. Can you clarify at 8:04 when you said each beads has many barcoded oligo-dT oligos on its surface. What is the scale of this "many"? Millions? Are these supposed to attach to the entire transcriptome of a cell?

  • @esan120au
    @esan120au 2 месяца назад

    17:13 quantifying proteins using DNA sequencing (CITE-Seq) 21:26 universal antibodies for a universal cell-surface protein that have unique barcodes for each cell to detect the unwanted doublets (droplet with one bead but two cells)

  • @dbs5817
    @dbs5817 2 месяца назад

    Awesome

  • @jameskellinger8314
    @jameskellinger8314 2 месяца назад

    What is the purpose of waving your hands?

  • @jameskellinger8314
    @jameskellinger8314 2 месяца назад

    Please don't wave your hands--it is very distracting

  • @mervatel-deftar8412
    @mervatel-deftar8412 2 месяца назад

    Amazing overview Excellent

  • @chepai1827
    @chepai1827 2 месяца назад

    Nice talk!

  • @JH-it2yr
    @JH-it2yr 2 месяца назад

    Very detailed and clear! No ads interrupting! Thank you very much professor!

  • @ylidene6222
    @ylidene6222 2 месяца назад

    Thank you! It helped me a lot

  • @user-il4jz8mu6o
    @user-il4jz8mu6o 3 месяца назад

    I looking for good videos in how analyzing the a dataset of Peripheral Blood Mononuclear Cells (PBMC) ?? Thank you

  • @1point689
    @1point689 3 месяца назад

    fourier transfer of fourier himself is such a nerd thing to do lol, love this lecture Thanks!

  • @1point689
    @1point689 3 месяца назад

    Great lecture!

  • @kalyanirajalingham1286
    @kalyanirajalingham1286 3 месяца назад

    Fantastic video! You explain very well

  • @user-ci7fe1jj8x
    @user-ci7fe1jj8x 3 месяца назад

    روى ابن ماجه وغيره أن النبي صلى الله عليه وسلم قال: إن مما يلحق المؤمن من عمله وحسناته بعد موته علما علمه ونشره، وولدا صالحا تركه، أو مصحفا ورثه، أو مسجدا بناه، أو بيتا لابن السبيل بناه، أو نهرا أجراه، أو صدقة أخرجها من ماله في صحته وحياته تلحقه بعد موته. m.ruclips.net/video/-qgsOvwlK0w/видео.html&pp=ygUg2KfZhdis2KfYr9mG2Kcg2LnZhdix2Ygg2YXYrNiv2Yk%3D

  • @exciton007
    @exciton007 3 месяца назад

    Thanks for this great presentation!

  • @loreenzo2120
    @loreenzo2120 3 месяца назад

    how does this not have more views

  • @prabhatmishras421
    @prabhatmishras421 3 месяца назад

    Best lecture

  • @thehumanconvergence
    @thehumanconvergence 3 месяца назад

    Great stuff, will be featuring you in our next episode!

  • @wilsonfrantine
    @wilsonfrantine 3 месяца назад

    If you are really interested on, u can dive in Jost 2018 (measuring differentiation). He argue about the limitations of Fst and its relative statistics (yes, there's a lot). You will see that comparing expected heterozygosity as a proxy of differentiation might fail at the populational level in several occasions. For instance, it can only reaches 1 if there are no allelic sharing, and put 11% of differentiation when there are 35% of allelic differences because it rely on the average of a possible heterozygosity that might comes from a loci in case of panmixing breeding (a lot of assumptions). Jost argue that it would be more helpful try to compare the frequency of alleles, mainly for those loci (or markers) that can measure a multialellic state ( more than two, usually). Fst still usefull, but you can't take that as the ultimate tool to measure everything. Remember, a 30 inch ruler is useless to measure the distance between the sun and the earth or the circumference of a cell.

  • @wemcal
    @wemcal 3 месяца назад

    Great video and great information

  • @aminchy01
    @aminchy01 3 месяца назад

    Great to hear

  • @thisumamarasinghe8171
    @thisumamarasinghe8171 3 месяца назад

    talking bullshit