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iBiology Techniques
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Добавлен 29 май 2012
Free biology talks by the world's leading scientists that show the process of science and the wonders of discovery. Please visit our other RUclips channels for more free videos:
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iBiology Career and Professional Development: Advice and training videos on how to navigate a career in science and beyond
Wonder Collaborative: A new initiative aimed to bring the stories of science to the general public
View our website at www.ibiology.org, and join our newsletter here: www.ibiology.org/join.html
iBiology: Seminars by the world's leading scientists
iBiology Science Stories: Stories of discovery and the series "How I became a scientist"
iBiology Career and Professional Development: Advice and training videos on how to navigate a career in science and beyond
Wonder Collaborative: A new initiative aimed to bring the stories of science to the general public
View our website at www.ibiology.org, and join our newsletter here: www.ibiology.org/join.html
Видео
Next Generation Sequencing 2: Illumina NGS Sample Preparation - Eric Chow (UCSF)
Просмотров 154 тыс.3 года назад
www.ibiology.org/techniques/next-generation-sequencing Next generation sequencing allows DNA samples to be sequenced quickly and affordably. Learn how next gen sequencing works and get tips on preparing and running your samples. In the past decade there has been an amazing change in the efficiency of DNA sequencing. Using traditional Sanger sequencing, the human genome project took 20 years and...
Single Cell Sequencing - Eric Chow (UCSF)
Просмотров 235 тыс.3 года назад
www.ibiology.org/techniques/single-cell-sequencing Dr. Eric Chow gives an overview of single cell sequencing, explains why this approach is useful, and talks through the leading methods. Single cell sequencing, as the name implies, allows researchers to examine the genomic information for individual cells. This provides an opportunity to examine cell-to-cell differences and identify cell subtyp...
Bioimage Analysis - Christian Tischer (EMBL)
Просмотров 12 тыс.4 года назад
www.ibiology.org/techniques/introduction-to-bioimage-analysis Dr. Christian Tischer walks us through the main concepts of a typical bioimage analysis workflow and explains how to quantitatively interpret the content of microscopy images. Microscopy is a key technology driving biological discovery. Nowadays, microscopy based scientific findings must be substantiated by quantitative image analysi...
Bioimage Analysis 1: The Basics: Getting Started (Anne Carpenter and Kevin Eliceiri)
Просмотров 9 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Image Acquisition for Quantitative Analysis - Nico Stuurman (UCSF)
Просмотров 10 тыс.4 года назад
www.ibiology.org/techniques/image-acquisition Nico Stuurman provides an overview of the different tools, equipment, and software available to acquire an image of a biological sample, and the considerations one needs to take when using these tools. How do we visualize biological samples? In this talk, Dr. Nico Stuurman provides an overview of the different tools, equipment, and software availabl...
Bioimage Analysis 2: Pre-Processing (Kevin Eliceiri)
Просмотров 10 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Bioimage Analysis 5: Measurement and Phenotype Classification (Anne Carpenter)
Просмотров 7 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Bioimage Analysis 4: Tracking (Kevin Eliceiri)
Просмотров 3,5 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Bioimage Analysis 3: Segmentation (Anne Carpenter)
Просмотров 34 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Bioimage Analysis 6: Tips and Best Practices (Anne Carpenter and Kevin Eliceiri)
Просмотров 2,8 тыс.4 года назад
www.ibiology.org/techniques/bioimage-analysis In this series of 6 videos, Dr. Anne Carpenter and Dr. Kevin Eliceiri provide an overview of bioimage analysis. Pre-processing is the first step that follows image acquisition and will prepare your image by reducing the signal-to-noise ratio, applying appropriate filters to the image, and color extraction. Once you perform pre-processing, you’re rea...
Multi-Dimensional Microscopy Datasets - Loic Royer (CZ Biohub)
Просмотров 4,2 тыс.4 года назад
www.ibiology.org/techniques/multi-dimensional-microscopy-datasets Modern microscopy produces large multi-dimensional datasets, which creates new challenges for data storage, processing and visualization. In this talk, Dr. Loic Royer uses a developing drosophila melanogaster embryo to explain how to solve some of the challenges produced by multi-dimensional microscopy datasets. Speaker Biography...
Metadata in BioImaging - Jason Swedlow (U. Dundee)
Просмотров 3,6 тыс.4 года назад
www.ibiology.org/techniques/metadata Jason Swedlow explains what constitutes image metadata, and provides examples on how to catalog, organize, analyze, and share the metadata of biological images. In order to understand an image of a biological sample and what it represents, one needs to understand its metadata. Metadata is the information behind the image that shows the experimental procedure...
iBiology Bioimage Analysis Course - Life cycle of an image data set
Просмотров 4,5 тыс.4 года назад
www.ibiology.org/online-biology-courses/bioimage-analysis-course This course is designed as a graduate student level introduction to bioimage analysis and will provide an overview of the practice and principles of microscopy digital image handling. This series follows the life cycle of an image data set, from acquisition to analysis. It teaches important concepts and best practices, and provide...
The Scientific Community Image Forum - Anne Carpenter and Kevin Eliceiri
Просмотров 5 тыс.4 года назад
www.ibiology.org/techniques/scientific-community-image-forum The Scientific Community Image Forum is an online resource that helps scientists answer their bioimage analysis questions. In this talk, Dr. Anne Carpenter and Dr. Kevin Eliceiri encourage scientists to use the Scientific Community Image Forum when they have image analysis difficulties, and to familiarize themselves with the different...
ImageJ - Kevin Eliceiri (U. Wisconsin)
Просмотров 14 тыс.4 года назад
www.ibiology.org/techniques/image-j In this talk, Dr. Kevin Eliceiri provides an overview of ImageJ, explains how ImageJ has evolved through time, and demonstrates major functionalities of this open-source software. Since 1987, different versions of ImageJ have been used by scientists to analyze biological images. Speaker Biography: Dr. Kevin Eliceiri is an Associate Professor of Medical Physic...
Video cuts off right when it was getting good
RIP roger. May your legacy continue to fluoresce.
Both the removal of scaffolding and the protein insertion machines as well as all the other unnamed irreducible complexity and interdependencies scream out Wonderfully Designed. Yet without any scientific evidence or explanation attributes their existence to the god of the evolutionary gaps, 'it evolved'. Has anyone ever explained a naturalistic process that could actually engineer this - or do we really have to rely on that oft repeated story?
Thank you very much for the tutorial! Can it be used for detecting cells in 3D images?
Isn't there a mistake on 9:27? I think the Fourier image should be rotated on 90 degrees, coz in the direction of x there are many freaquences due to sharp steps.
Very interesting about nothing.
great video. I needed this!
sir i need a little help in selecting probe for my research can u please help me by ur kind suggestion
FACS sorting hehe
Great explanation professor
The very first virus
This was crummy. There are many logical gaps in the presentation. E.g., it is not clear how clusters have the same sequence. There are other RUclips videos on NGS that are clearer.
Great explanation 👌
I found this video very interesting. I have experience with mass spectrometer (LCMS) but this was interesting as it uses a similar principle.
kon kon class 8 me h or kis kis ko ye video goggle se mila h like karo
What a revelation! That Huygens figured out the fundamentals in 1690 is also mind-blowing. Thank you for this fascinating presentation. It utterly changed the way I think about light and optics.
Can someone help me? I read a study that said they "looked at the various forms of the gene coding for a vasopressin receptor" among various people. Does that mean they only looked at active genes, or inactive ones as well?
How can you quantitate your images is vitally important because cellular processes are governed by the laws of physics. To help people with biology we need a physically accurate picture of the cell. Fluorescence excitation causes changes in the eigenstate of the electronic energy levels of the excited molecules, which can result in radiation that changes the state of neighboring molecules. However, fluorescence excitation still remains a powerful tool for measuring the rates of cellular processes. Dr. Dray always concludes her videos with sunscreen and subscribe because all humans are in a race against time to slow down the dynamics of radiation causing protein and DNA mutation in their cells.
Skip to 14:00 if you just care about microscope
I just want to say that you're a great teacher! Passing on not only knowledge but also the passion!
Thanks a lot to give this presentation!
How do i import images on cellprofiler and how can i get a software that works on windows 7
Thanks a lot. What would happen if instead of putting 5% PhiX in the cartridge, I mistakenly put more amount (60%). And I put the correct amount from the library?
amazing lifesaver
Thanks so much! Even the illumina webinar was not as clear as your presentation! 👏👏👏👏
Thank you for the great explaination!
this is great!
Is it safe then to assume that molecules that divide would be the point at which inorganic becomes organic and chemistry becomes biology?
Thanks. Can you clarify at 8:04 when you said each beads has many barcoded oligo-dT oligos on its surface. What is the scale of this "many"? Millions? Are these supposed to attach to the entire transcriptome of a cell?
17:13 quantifying proteins using DNA sequencing (CITE-Seq) 21:26 universal antibodies for a universal cell-surface protein that have unique barcodes for each cell to detect the unwanted doublets (droplet with one bead but two cells)
Awesome
What is the purpose of waving your hands?
Please don't wave your hands--it is very distracting
Amazing overview Excellent
Nice talk!
Very detailed and clear! No ads interrupting! Thank you very much professor!
Thank you! It helped me a lot
I looking for good videos in how analyzing the a dataset of Peripheral Blood Mononuclear Cells (PBMC) ?? Thank you
fourier transfer of fourier himself is such a nerd thing to do lol, love this lecture Thanks!
Great lecture!
Fantastic video! You explain very well
روى ابن ماجه وغيره أن النبي صلى الله عليه وسلم قال: إن مما يلحق المؤمن من عمله وحسناته بعد موته علما علمه ونشره، وولدا صالحا تركه، أو مصحفا ورثه، أو مسجدا بناه، أو بيتا لابن السبيل بناه، أو نهرا أجراه، أو صدقة أخرجها من ماله في صحته وحياته تلحقه بعد موته. m.ruclips.net/video/-qgsOvwlK0w/видео.html&pp=ygUg2KfZhdis2KfYr9mG2Kcg2LnZhdix2Ygg2YXYrNiv2Yk%3D
Thanks for this great presentation!
how does this not have more views
Best lecture
Great stuff, will be featuring you in our next episode!
If you are really interested on, u can dive in Jost 2018 (measuring differentiation). He argue about the limitations of Fst and its relative statistics (yes, there's a lot). You will see that comparing expected heterozygosity as a proxy of differentiation might fail at the populational level in several occasions. For instance, it can only reaches 1 if there are no allelic sharing, and put 11% of differentiation when there are 35% of allelic differences because it rely on the average of a possible heterozygosity that might comes from a loci in case of panmixing breeding (a lot of assumptions). Jost argue that it would be more helpful try to compare the frequency of alleles, mainly for those loci (or markers) that can measure a multialellic state ( more than two, usually). Fst still usefull, but you can't take that as the ultimate tool to measure everything. Remember, a 30 inch ruler is useless to measure the distance between the sun and the earth or the circumference of a cell.
Great video and great information
Great to hear
talking bullshit